Detailed view for PF3D7_0801500

Basic information

TDR Targets ID: 1067
Plasmodium falciparum, nucleolar protein 10, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.9986 | Length (AA): 549 | MW (Da): 64409 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
52 335 1got (B) 53 340 11.00 0 1 0.71 -0.25
85 377 2h14 (A) 33 331 12.00 0 0.85 0.72 -0.3
4 293 3fm0 (A) 23 331 6.00 0.000000058 0.67 0.462133 0.42
52 340 4ery (A) 43 334 12.00 0 1 0.738312 -0.23
175 255 4o9d (B) 531 625 33.00 0.033 0.3 0.455341 -0.13
406 539 4uos (A) 1 139 19.00 0.31 0.2 0.49698 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Ring. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, sporozoite, early ring, early trophozoite, late ring. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late trophozoite, Oocyst, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Male Gametocyte. Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_128058)

Species Accession Gene Product
Arabidopsis thaliana AT3G56990   embryo sac development arrest 7 protein
Babesia bovis BBOV_II003390   hypothetical protein
Brugia malayi Bm1_37975   protein F32E10.1
Candida albicans CaO19_6686   hypothetical protein
Candida albicans CaO19.13978   potential WD40 domain
Candida albicans CaO19.6686   potential WD40 domain
Caenorhabditis elegans CELE_F32E10.1   Protein NOL-10
Cryptosporidium parvum cgd1_3270   hypothetical protein
Dictyostelium discoideum DDB_G0292466   NUC153 domain-containing protein
Drosophila melanogaster Dmel_CG7516   lethal (2) 34Fd
Echinococcus granulosus EgrG_001033400   nucleolar protein 10
Entamoeba histolytica EHI_141370   nucleolar protein 10, putative
Echinococcus multilocularis EmuJ_001033400   nucleolar protein 10
Giardia lamblia GL50803_13616   Glycine-rich protein
Homo sapiens ENSG00000115761   nucleolar protein 10
Leishmania braziliensis LbrM.26.1270   hypothetical protein, conserved
Leishmania donovani LdBPK_261230.1   hypothetical protein, conserved
Leishmania infantum LinJ.26.1230   hypothetical protein, conserved
Leishmania major LmjF.26.1250   hypothetical protein, conserved
Leishmania mexicana LmxM.26.1250   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02938   nucleolar protein 10
Mus musculus ENSMUSG00000061458   nucleolar protein 10
Neospora caninum NCLIV_002430   hypothetical protein, conserved
Oryza sativa 4339985   Os06g0128400
Plasmodium berghei PBANKA_1228700   nucleolar protein 10, putative
Plasmodium falciparum PF3D7_0801500   nucleolar protein 10, putative
Plasmodium knowlesi PKNH_0117400   nucleolar protein 10, putative
Plasmodium vivax PVX_093665   hypothetical protein, conserved
Plasmodium yoelii PY04189   Arabidopsis thaliana At3g56990/F24I3_70-related
Saccharomyces cerevisiae YGR145W   Enp2p
Schistosoma japonicum Sjp_0218790   Nucleolar protein 10, putative
Schistosoma mansoni Smp_180370   hypothetical protein
Schmidtea mediterranea mk4.004327.03   Nucleolar protein 10
Schmidtea mediterranea mk4.003143.01   Nucleolar protein 10
Trypanosoma brucei gambiense Tbg972.7.620   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.700   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509543.40   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511257.20   hypothetical protein, conserved
Toxoplasma gondii TGME49_295320   embryo sac development arrest EDA7, putative
Theileria parva TP04_0248   hypothetical protein, conserved
Trichomonas vaginalis TVAG_139260   nucleolar protein, putative

Essentiality

PF3D7_0801500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.700 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.700 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.700 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.700 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F32E10.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F32E10.1 Caenorhabditis elegans larval arrest wormbase
CELE_F32E10.1 Caenorhabditis elegans slow growth wormbase
YGR145W Saccharomyces cerevisiae inviable yeastgenome
TGME49_295320 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0801500 (Plasmodium falciparum), nucleolar protein 10, putative
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